
Description
Lipofectamine®LTXReagentisaplasmidtransfectionreagentthatofferstherightbalanceofpotencyandgentlenessforyourcells,resultinginthehighestpossIBLetransfectionefficienciesandviABIlities.Lipofectamine®LTXReagentisanadvancedsolutionforgeneexpressioninbothcommonandchallengingcelltypes.NootherplasmidDNA-specifictransfectionreagentcanmatchtheefficiency,convenience,andgentlenessofLipofectamine®LTXReagentfortransfectionofprimary,challenging,andsensitivecelllines.Lipofectamine®LTXReagentissynthesizedfrom100%animalorigin-freecomponents,makingiteasytovalidatetheabsenceofzoonoticdiseases,suchasBSEorviruses,inresearchexperimentsorcelllines.Lipofectamine®LTXReagentoffers:
•Superiordelivery—transfectabroadrangeofcelltypes,includingprimary,challenging,andsensitivecells,withhighefficiency
•Superiorperformance—combinehightransfectionefficiencywithmaximal(>90%)cellviability
•Optimizedprotocols—rapid,simpleprotocolsprovidedformostcelllines
•Ahighlyeffectivereagenttogeneratestablecelllines
Transfectprimarycellsefficiently
Primarycellsareoftendifficulttotransfect.Lipofectamine®LTXReagent,whencombinedwithPLUS™Reagent,providesthehighestleveloftransfectioninprimarycellsofanylipid-orpolymer-basedreagent.ManyprimarycelltypeshavebeensuccessfullytransfectedwithLipofectamine®LTXReagent,includingprimaryneuralProgenitorcells.
Superiordeliveryintochallengingcells
WhenitcomestoachievinghighlevelsofproteinexpressioninRAW267.3macrophages,Lipofectamine®LTXReagentoutperformscompetingtransfectionreagents(seefigure).Achievinghigh-leveltransgeneexpressionincellsthataretrADItionallyrefractorytotransfectionallowsyoutoobtainresultsthatwerepreviouslyunattainable.
Broadcompatibilitywithcelllines
Inadditiontobeingexcellentfortransfectingprimaryandchallengingcells,Lipofectamine®LTXReagentprovidesyouwiththemostversatileapproachtotransfectingestablishedcellslines,includingthosethataresensitivetoothertransfectionreagents.UseourTransfectionSelectionTooltohelpyouselectthebesttransfectionconditionsforthecellswithwhichyouwork.
Stabletransfection
Lipofectamine®LTXstrikestherightbalancebetweenpotencyandgentleness,givingyouthehighestpossiblepercentageofsuccessfullytransfectedcells.ThismakesLipofectamine®LTXReagentthequickestandeasiestreagentforestablishingstabletransfectantsfromchallengingorrefractorycelltypes.
Simple,rapid,serum-compatibleprotocol
Lipofectamine®LTXReagentoffersastreamlinedprotocol—noneedtoremovetransfectioncomplexesorchange/addmediumfollowingtransfection.Asimpleoptimizationprotocolhelpsyougetthebestresultsquickly,soyou"llspendlesshands-ontime,andgetfasterresults.
Figures


ExamplesofLipofectamine®LTXtransfection

Lipofectamine®LTXtransfectionefficiency

Lipofectamine®LTXReagentcombineshightransfectionefficiencywithlowcytotoxicitysuperiortoFuGENE®6andFuGENE®HDtransfectionreagents

TransfectionoptimizationofHumanMesenchymalStemCells(hMCS)

Plasmidtransfection.

Lipofectamine®LTXinthepresenceofPLUS™Reagentisanexcellentchoicefortransfectionofhumancancercelllinesaswellasprimaryandstemcells.Thereagentenablesthedeliveryofhightransfectionperformancewithoutassociatedcytotoxicity

Lipofectamine®LTXReagent,withPlus™Reagent,efficientlytransfectsprimaryneuralprogenitorcells

Asimple,rapid,serum-compatibleprotocol

Lipofectamine®LTXtransfectionprotocol.

Lipofectamine®LTXReagent,withPLUS™Reagent,efficientlytransfectsprimaryneuralprogenitorcells

Lipofectamine®LTXTransfectionProcedureDetails

Lipofectamine®LTXTransfectionWorkflow

Specifications
CellType: | EstablishedCellLines, Hard-to-TransfectCells, PrimaryCells, StemCells |
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SampleType(Specific): | PlasmidDNA, RNAiPlasmids(shRNA,miR) |
Classification: | AnimalOrigin-Free |
TransfectionTechnique: | Lipid-BasedTransfection |
SerumCompatible: | Yes |
HighThroughputCompatibility: | HighThroughput-Compatible |
ProductLine: | Lipofectamine® |
ProductSize: | 1mL |
ShippingCondition: | WetIce |