
Description
Lipofectamine®LTXReagentisaplasmidtransfectionreagentthatofferstherightbalanceofpotencyandgentlenessforyourcells,resultinginthehighestpossIBLetransfectionefficienciesandviABIlities.Lipofectamine®LTXReagentisanew,advancedsolutionforgeneexpressioninbothcommonandchallengingcelltypes.NootherplasmidDNA-specifictransfectionreagentcanmatchtheefficiency,convenience,andgentlenessofLipofectamine®LTXReagentfortransfectionofprimary,challenging,andsensitivecelllines.Lipofectamine®LTXReagentissynthesizedfrom100%animalorigin-freecomponents,makingiteasytovalidatetheabsenceofzoonoticdiseases,suchasBSEorviruses,inresearchexperimentsorcelllines.AcertificateoforiginmaybeobtainedbycontactingTechnicalSupport.Lipofectamine®LTXReagentoffers:
•Superiordelivery–transfectabroadrangeofcelltypes,includingprimarychallengingandsensitivecells,withhighefficiency
•Superiorperformance–combinehightransfectionefficiencywithmaximal(>90%)cellviability
•Optimizedprotocols–rapid,simpleprotocolsprovidedformostcelllines
•Ahighlyeffectivereagenttogeneratestablecelllines
TransfectPrimaryCellsEfficiently
Primarycellsareoftendifficulttotransfect.Lipofectamine®LTXReagent,whencombinedwithPLUS™Reagent,providesthehighestleveloftransfectioninprimarycellsofanylipid-orpolymer-basedreagent.ManyprimarycelltypeshavebeensuccessfullytransfectedwithLipofectamine®LTXincludingprimaryneuralProgenitorcells.
SuperiorDeliveryintoChallengingCells
WhenitcomestoachievinghighlevelsofproteinexpressioninRAW267.3macrophages,Lipofectamine®LTXReagentoutperformscompetingtransfectionreagents(seeFigure4).Achievinghigh-leveltransgeneexpressionincellsthataretrADItionallyrefractorytotransfectionallowsyoutoobtainresultsthatwerepreviouslyunattainable.
BroadCompatibilitywithCellLines
Inadditiontobeingexcellentfortransfectingprimaryandchallengingcells,Lipofectamine®LTXReagentprovidesyouwiththemostversatileapproachtotransfectingestablishedcellslines,includingthosethataresensitivetoothertransfectionreagents.UseourTransfectionSelectionTooltohelpyouselectthebesttransfectionconditionsforthecellswithwhichyouwork.
StableTransfection
Lipofectamine®LTXstrikestherightbalancebetweenpotencyandgentleness,givingyouthehighestpossiblepercentageofsuccessfullytransfectedcells.ThismakesLipofectamine®LTXthequickestandeasiestreagentforestablishingstabletransfectantsfromchallengingorrefractorycelltypes.
Simple,Rapid,Serum-compatibleProtocol
Lipofectamine®LTXReagentoffersastreamlinedprotocol–noneedtoremovetransfectioncomplexesorchange/addmediumfollowingtransfection.Asimple,optimizationprotocolhelpsyougetthebestresultsquicklysoyou"llspendlesshands-ontime,andgetfasterresults.
Lipofectamine®LTXandPLUS™Reagentisavailableinthefollowingsizes:
•0.1mlwith40µlPLUS™Reagent
•0.3mlwith0.25mlPLUS™Reagent
•1.0mlwith0.85mlPLUS™Reagent
•15ml(purchasePLUS™Reagentseparately)
Figures


Lipofectamine®LTXtransfectionefficiency

Lipofectamine®LTXReagentcombineshightransfectionefficiencywithlowcytotoxicitysuperiortoFuGENE®6andFuGENE®HDtransfectionreagents

TransfectionoptimizationofHumanMesenchymalStemCells(hMCS)

Plasmidtransfection.

Lipofectamine®LTXinthepresenceofPLUS™Reagentisanexcellentchoicefortransfectionofhumancancercelllinesaswellasprimaryandstemcells.Thereagentenablesthedeliveryofhightransfectionperformancewithoutassociatedcytotoxicity

Lipofectamine®LTXReagent,withPlus™Reagent,efficientlytransfectsprimaryneuralprogenitorcells

Asimple,rapid,serum-compatibleprotocol

Lipofectamine®LTXtransfectionprotocol.

Lipofectamine®LTXReagent,withPLUS™Reagent,efficientlytransfectsprimaryneuralprogenitorcells

Lipofectamine®LTXTransfectionProcedureDetails

Lipofectamine®LTXTransfectionWorkflow

Specifications
CellType: | EstablishedCellLines, Hard-to-TransfectCells, PrimaryCells, StemCells |
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SampleType(Specific): | PlasmidDNA, RNAiPlasmids(shRNA,miR) |
Classification: | AnimalOrigin-Free |
TransfectionTechnique: | Lipid-BasedTransfection |
SerumCompatible: | Yes |
HighThroughputCompatibility: | HighThroughput-Compatible |
ProductLine: | Lipofectamine® |
ProductSize: | 15ml |
ShippingCondition: | WetIce |