Gibco/FreeStyle 293表达培养基/6 x 1 L/12338026
商品编号：
12338026
市场价：
¥12160.00
美元价：
7296.00
产品分类：
培养基
公司分类：
culture_medium
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description
FreeStyle™293ExpressionMediumisananimalorigin–free,chemicallydefined,protein-freemediumspecificallydevelopedtosupportthegrowthandtransfectionof293-FcellsinsUSPensionwithoutadaptation.Themediumiscompleteandready-to-use,withnosupplementationrequired.Gibco®FreeStyle™293ExpressionMediumfeatures:•Completeandready-to-useformatcontainingGlutaMAX™supplement
•ABIlitytosupportsuspensiongrowthandtransfectionof293-Fcells
•Animalorigin–free,protein-freeandchemicallydefinedformulation
•EasyusewiththeFreeStyle™293Expressionsystems
•Scalabilityinspinnerflasksandbioreactors
Completeandready-to-useformatcontainingGlutaMAX™supplement
Gibco®FreeStyle™293ExpressionMediumisreadytouse.Itdoesnotrequiretheadditionofserum,glutamine,orsurfactants.Gibco®FreeStyle™293ExpressionMediumcontainsGlutaMAX™supplementtominimizetoxicammoniabuild-upandimprovecellviabilityandgrowthinaneasy-to-useformat.
Abilitytosupportsuspensiongrowthandtransfectionof293-Fcells
Gibco®293-F,293-H,andFreeStyle™293-FcellsinsuspensioncanbecultureddirectlyintoGibco®FreeStyle™293ExpressionMediumwithoutadaptation.Cellsadaptedtoothercommerciallyavailableserum-freemediacanbesubcultureddirectlyintoGibco®FreeStyle™293ExpressionMedium,usuallywithoutanyfurtheradaptation.Cellsusuallyrequireadaptationfromserum-containingformulations.CellsculturedinGibco®FreeStyle™293ExpressionMediumcanbetransfectedusinglipidbasedtransfectionreagents,suchas293fectin™reagentandFreestyle™MAXreagent.
Animalorigin–free,protein-freeandchemicallydefinedformulation
Gibco®FreeStyle™293ExpressionMediumisanimalorigin–free,protein-freeandchemicallydefined,allowingforeasierpurificationofyourproteinofinterest.Gibco®chemicallydefinedmediacontainnoproteins,hydrolysates,orcomponentsofunknowncomposition.
EasyuseaspartoftheFreeStyle™293ExpressionSystemandFreeStyle™MAXExpressionSystemforproteinproduction
TheFreeStyle™293ExpressionSystemisacomplete,suspensioncellculturesystemforgeneratinglargeamountsofmammalianrecombinantprotein(seefigure).TheFreeStyle™293ExpressionSystemcombinesGibco®FreeStyle™293ExpressionMediumwith293fectin™reagent,acationic-lipidformulationdesignedtotransfectsuspensionFreeStyle™293-Fcellsathighefficiency.TheFreeStyle™MAXExpressionSystemisabreakthroughtechnologyforrapidandhigh-yieldmammalianproteinproduction.TheFreeStyle™MAXExpressionSystemcombinesGibco®FreeStyle™Media,FreeStyle™MAXReagent,andeitherFreeStyle™CHO-ScellsorFreeStyle™293-Fcells.
Scalabilityinspinnerflasksandbioreactors
ProteinproductionusingGibco®FreeStyle™293ExpressionMediumcanbescaledupinspinnerflasksorbioreactors.Theappropriatespinnerorimpellerspeedandseedingdensityshouldbeoptimizedforeachsystem.Dependingontheimpellerdesignandspeed,itmaybenecessarytosupplementGibco®FreeStyle™293ExpressionMediumwithadditionalPluronic®F-68toavoidsheerstressintheculture.
Universalbagpackaging
Gibco®universalbagsofferflexIBLepackagingthatallowsyoutointegrateasingle-use,disposablelargevolumemediacontainerwithawidevarietyofautomatedcellcultureinstruments,disposablebioreactorsystems,andsterileconnectionprocesses,minimizingtheneedforcostlycustomization.
Productuse
CustomersusingGibco®FreeStyle™293ExpressionMediuminamanufacturingprocess,whohaveasubmissionwiththeFDA,mayrequestaletterofauthorizationfromustoreferenceourTypeIIDrugMasterFile(DMF).
cGMPmanufacturingandqualitysystem
Gibco®FreeStyle™293ExpressionMediumismanufacturedatacGMP-compliantfacilitylocatedinGrandIsland,NewYork.ThefacilityisregisteredwiththeFDAasamedicaldevicemanufacturerandiscertifiedtoISO13485standards.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
Figures
ComparableexpressioninFreeStyleâ„¢293-FandF17Cells
ComparabletransfectionofFreeStyleâ„¢293-FandF17Cells
ProteinproducedfromtransientFreeStyleâ„¢293-FCelltransfection
Specifications
| CellLine: | FreeStyle™293-FCells |
|---|---|
| Classification: | AnimalOrigin-Free, ChemicallyDefined, Protein-Free, Serum-Free |
| CultureType: | SuspensionCellCulture |
| Form: | Liquid |
| Glutamine: | GlutaMAX™-I |
| ProductLine: | Freestyle™, Gibco® |
| ProductSize: | 1,000mL |
| SerumLevel: | Serum-Free |
| GreenFeatures: | Sustainablepackaging |
| ShippingCondition: | RoomTemperature |
Contents&storage
Storageconditions:2-8°C.Protectfromlight
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
Shippingconditions:Ambient
Shelflife:12monthsfromdateofmanufacture
品牌介绍
GIBCO®是Thermofisher公司的一个重要品牌，1962年诞生于美国纽约的大岛(Grand Island)。致力于细胞培养产品的研发和生产，是世界上最早也是最大的细胞培养产品的供应商，是细胞培养领域全球标准的制定者。Gibco®血清凭借其一致的品质、超级可靠性和广受赞誉的支持服务，赢得全球研究人员的一致信任。Gibco®血清可满足您的研究需求及预算控制需要，为您最大限度挖掘基础细胞培养、专业研究和特殊试验的价值。我们致力于为您的特定细胞培养需求和实验室预算控制需要提供最适宜的胎牛血清(FBS)产品。Gibco产品拥有 50 年历史，将科学与经验进行融合从而重新定义了细胞培养的可能性。Gibco提供了一系列创新型高性能产品和基于专业知识的服务，包括快速灵活的细胞系开发、培养基和补料、一流的技术、高质量的定制培养基开发和培养基优化服务。 Gibco作为细胞培养领域值得信赖的品牌，持续在行业内树立高基准。
品牌分类
肝实质细胞
2-巯基乙醇
F-12营养液
盖尔特基底膜
哺乳动物表达试剂盒
锥虫
细胞外基质蛋白
水
其他血液的血清
通用瓶
厄尔平衡盐溶液
DMEM媒体
细胞外基质涂层板
汉克斯平衡盐溶液
其他细胞解离试剂
293媒体
低温保存介质
DMEM F-12媒体
最小基本介质（MEM）
转染试剂
胰蛋白酶
Iscove公司（IMDM）
藻基质生物咖啡
杜尔贝科磷酸盐缓冲盐
其他无血清专业媒体
其他一般化学品
一加转染试剂
生长因子，趋化因子细胞因子
电穿孔仪配件
抗生素抗真菌药
RPMI介质1640
HEPES缓冲液
神经细胞培养基
胎牛血清
血清置换
胰岛素，转铁蛋白和铁补充剂
其他补充剂
干细胞分化试剂盒
磷酸盐缓冲盐碱
电穿孔仪器
CHO DG44媒体
哺乳动物细胞系
莱博维茨L-15
施耐德果蝇媒体
显示分类
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