Gibco/MEM, NEAA, powder/10 L/41500018

来自 : 发布时间：2024-05-01

Description

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With	Without
• NEAA	• HEPES
• L-glutamine	• Sodium Bicarbonate
• Phenol Red

The complete formulation is available.Gibco® MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO₂ incubator, or with Hanks salts for use without CO₂. This product is made with Earle’s salts.Product Intended UseFor in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.cGMP Manufacturing and Quality SystemFor supply chain continuity, we manufacture Gibco® MEM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).For In Vitro Diagnostic Use.

Specifications

Glutamine:Phenol Red Indicator:Form:HEPES Buffer:Sodium Pyruvate Additive:Glucose:Product Line:Product Size:Serum Supplementation:Shipping Condition:

L-Glutamine

Phenol Red

Powder

No HEPES

No Sodium Pyruvate

Low Glucose

Gibco®

10 L

Standard Serum Supplementation

Room Temperature

Contents & storage

Storage conditions:2-8° CShipping conditions: AmbientShelf life: 36 months from date of manufacture

Media formulations

41500 - MEM, powder, NEAA