Gibco/DMEM/F-12, powder/50 L/12500096

来自 : 发布时间：2025-04-03

Description

Dulbeccos Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco® DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM / F-12 is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red	• Sodium Bicarbonate

The complete formulation is available.Gibco® DMEM / F-12 is a 1:1 mixture of DMEM and Hams F-12. This formulation combines DMEMs high concentrations of glucose, amino acids and vitamins with F-12s wide variety of components.Product Intended UseFor in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.cGMP Manufacturing and Quality SystemGibco® DMEM / F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® DMEM / F-12 product made in our Scotland facility (32500-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO₂ environment to maintain physiological pH.Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).For In Vitro Diagnostic Use.

Specifications

Glutamine:Phenol Red Indicator:Form:HEPES Buffer:Sodium Pyruvate Additive:Serum Supplementation:Glucose:Product Line:Product Size:Shipping Condition:

L-Glutamine

Phenol Red

Powder

No HEPES

Sodium Pyruvate

Standard Serum Supplementation

High Glucose

Gibco®

10 x 1 L

Room Temperature

Contents & storage

Storage conditions:2-8° CShipping conditions: AmbientShelf life: 36 months from date of manufacture

Media formulations

12500 - DMEM/F-12, powder